| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211872 |
| Chromosome: | chromosome_6 |
| Location: | 3826482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278170 | FAP237,FAS7 | Fasciclin-like protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTATGCTGCTGTATGGCGCCCCTTCCTATG |
| Internal bar code: | CTTTGAAGGGGCTATGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 99.5117 |
| LEAP-Seq n confirming: | 5706 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCAGACCAACAACCTA |
| Suggested primer 2: | CCTGCCCACCTTACATGTCT |