Insertion junction: LMJ.RY0402.211877_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211877
Chromosome:chromosome_2
Location:1395527
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g083354 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GTCTTCCATCCCGGCCTGCCTGTCACACAG
Internal bar code:AACGGTGGTTGGATGCGGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:586
LEAP-Seq percent confirming:97.1638
LEAP-Seq n confirming:3837
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGTTTGCGTACCGTTCC
Suggested primer 2:ACTCCATGGGTAGTGCGAAG