| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211878 |
| Chromosome: | chromosome 11 |
| Location: | 1663598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467774 | (1 of 1) IPR000104//IPR003409 - Antifreeze protein, type I // MORN motif | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCAACGCCACCTGCCCATTGGCAACA |
| Internal bar code: | AAAAATAACGACTGGTCCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.9528 |
| LEAP-Seq n confirming: | 4232 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCAAGTTACGACCACGC |
| Suggested primer 2: | TGATCGTTGATGAAGCGAAG |