| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211920 |
| Chromosome: | chromosome 12 |
| Location: | 7351861 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557100 | (1 of 3) K00573 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (E2.1.1.77, pcm) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACGTCGGGAGGACAACCTCTAAACACG |
| Internal bar code: | GGGTTATTGGTTTGATGAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.664 |
| LEAP-Seq n confirming: | 5636 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCCTGATTTGTTACTGG |
| Suggested primer 2: | ACACATCAGCAGCGTTTCAG |