| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211921 |
| Chromosome: | chromosome 12 |
| Location: | 8417490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548651 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTACTGCCGCGCCCGCACTGGCTGCTGC |
| Internal bar code: | CTTATTCGAGTGCGCCGCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.7219 |
| LEAP-Seq n confirming: | 24382 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAAGAAGGCAGCAAAGT |
| Suggested primer 2: | GACTTGAGCAACCTAAGCCG |