| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211924 |
| Chromosome: | chromosome 17 |
| Location: | 4111433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729500 | (1 of 1) PTHR22870//PTHR22870:SF136 - REGULATOR OF CHROMOSOME CONDENSATION // ALSIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCTGCCAGCCGCAATGCCAACCACGTC |
| Internal bar code: | ACTGAGACACAGATAGAGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 92.685 |
| LEAP-Seq n confirming: | 2230 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGACCTTGTTGGACAT |
| Suggested primer 2: | ATCCTGAACCAAACAGGTCG |