| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211934 |
| Chromosome: | chromosome 12 |
| Location: | 245415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485300 | CSV6 | Chlamydomonas-specific family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCACTGCTGAACGGTGACGGTGGGGT |
| Internal bar code: | CTGTATCGATGAGGATGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 94.302 |
| LEAP-Seq n confirming: | 993 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAAGTGAGCTGTTGAG |
| Suggested primer 2: | GGAGCACGACGACATTGAC |