| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211944 |
| Chromosome: | chromosome 9 |
| Location: | 3215984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387578 | (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGAGGCTCATTGTGCGGGTGAGGCG |
| Internal bar code: | AGTGCTTGGACCCACGCCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.0739 |
| LEAP-Seq n confirming: | 5670 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTGCTGTCTCTGGCCTT |
| Suggested primer 2: | AGTTTAACGGAAACGCGAGA |