| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211952 |
| Chromosome: | chromosome 3 |
| Location: | 6753426 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198100 | BET1 | ER-to-Golgi Qc-SNARE protein, Bet1/mBET1 family (Qc.II); (1 of 1) K08504 - blocked early in transport 1 (BET1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGAACATAAATTACTACATAGCGTCATA |
| Internal bar code: | TATGGATGTCCGGTTGGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.7499 |
| LEAP-Seq n confirming: | 6382 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGAAGCACGCCAGTAT |
| Suggested primer 2: | TCCCGTGTGTTACCTGCATA |