| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211966 |
| Chromosome: | chromosome 10 |
| Location: | 1856972 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431350 | TGL16 | (1 of 4) PTHR12406//PTHR12406:SF27 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED // SUBFAMILY NOT NAMED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTCTTGGCCGGGCAAAGGGGCAAATGT |
| Internal bar code: | GGCCGGACCGGGTTATTAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.9022 |
| LEAP-Seq n confirming: | 2043 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACCTACGCCCTGTTTC |
| Suggested primer 2: | CGTGTGATATAATTCCCGCC |