| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211979 |
| Chromosome: | chromosome 7 |
| Location: | 4577965 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344000 | (1 of 1) PTHR31988:SF1 - 9-O-ACETYL-N-ACETYLNEURAMINIC ACID DEACETYLASE-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGACCTTAAATGACATGGGCTGTAGCG |
| Internal bar code: | GCTCGTTTTACGAGTAGAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.5708 |
| LEAP-Seq n confirming: | 8120 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTCGTATAGCCTGCAC |
| Suggested primer 2: | CATACACATACTGGCCGCAC |