Insertion junction: LMJ.RY0402.211984_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211984
Chromosome:chromosome 10
Location:3980575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g448750 GT90F21,GT90-21 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 21 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGAAGTACCACTGGTTGTTGCGGACCA
Internal bar code:CGTTGCTTGTACAACTTAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:100
LEAP-Seq percent confirming:99.2395
LEAP-Seq n confirming:261
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTGGCTGTATGGAGTCAT
Suggested primer 2:CATGGCTCATCCCACTTTTT