| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212016 |
| Chromosome: | chromosome 7 |
| Location: | 2317537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGCAATGCTGTGAGCCTGTGATATGG |
| Internal bar code: | TGGTAGTGCTTCTCGGTATGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 99.5442 |
| LEAP-Seq n confirming: | 6552 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGGAAATCGAGTACGTG |
| Suggested primer 2: | ACCATCTCGTCATCTACGCC |