| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212020 |
| Chromosome: | chromosome 12 |
| Location: | 7360623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557050 | CPLD8,YGG2 | Conserved expressed protein with YGGT domain; (1 of 2) K02221 - YggT family protein (yggT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGATATGCGGGTTTGGAGAAGGGAAGATG |
| Internal bar code: | AGCCAGTCGGTTGCACGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.6382 |
| LEAP-Seq n confirming: | 22857 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGAACTTGACGTGGTTG |
| Suggested primer 2: | CCTGTACAGACCCGTCCACT |