Insertion junction: LMJ.RY0402.212033_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212033
Chromosome:chromosome 4
Location:493217
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCATGGGGGGATCTGGGGTGTGCCTGGA
Internal bar code:ACGGTTTCGTTTTCGACTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:673
LEAP-Seq percent confirming:98.7115
LEAP-Seq n confirming:6052
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:TAACATTCCTAGGGGCGATG
Suggested primer 2:GTGCAAAAGCTCAAGGAAGG