| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212050 |
| Chromosome: | chromosome 10 |
| Location: | 1240024 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426650 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAACGTGCCGGCACGGGCTGGTGGGGG |
| Internal bar code: | ATCGGCGAGCGGGCTTTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.7546 |
| LEAP-Seq n confirming: | 4471 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGAGCAGTGGTCTGAAG |
| Suggested primer 2: | CAGCGTACACGTCCTCAAAA |