| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212061 |
| Chromosome: | chromosome 8 |
| Location: | 3954222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378650 | CGl131 | (1 of 18) IPR011051 - RmlC-like cupin domain; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATTGTGGTGTCACTGCTGTGGCGCCCTG |
| Internal bar code: | GTTTATGAATATGGTGTTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 99.6634 |
| LEAP-Seq n confirming: | 27540 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAACCAAGGGTGGGAT |
| Suggested primer 2: | TCTAGCTGGTGTGGCTGATG |