| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212064 |
| Chromosome: | chromosome 3 |
| Location: | 3843501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170700 | (1 of 3) 3.2.1.39 - Glucan endo-1,3-beta-D-glucosidase / Laminarinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGAGGAATTTCAGCTATCGCAGCCTTTG |
| Internal bar code: | TGAGCGTCGCAACACAACGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1070 |
| LEAP-Seq percent confirming: | 99.3491 |
| LEAP-Seq n confirming: | 4121 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATATTATCATCGGCGCTT |
| Suggested primer 2: | CACACCAAACACACACTCCC |