| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212070 |
| Chromosome: | chromosome 6 |
| Location: | 1220901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGGCGGGGAAACGTACGTGCGGAACT |
| Internal bar code: | GGGGGAGATCCCAATGCACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 97.4651 |
| LEAP-Seq n confirming: | 4191 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTACGACAGTGCCCAAC |
| Suggested primer 2: | TGCTAACATGTCGAGTTGCC |