| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212070 |
| Chromosome: | chromosome 6 |
| Location: | 1220901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGTCGAGTGGCGCAGCCCACCGAGCC |
| Internal bar code: | GCCCGAGTAGGCACGGTTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTACGACAGTGCCCAAC |
| Suggested primer 2: | TGCTAACATGTCGAGTTGCC |