Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.212090 |
Chromosome: | chromosome_5 |
Location: | 765208 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g248500 | Mitochondrial thioredoxin o | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGGCTGCGGGGGCATGTTGGTGGCGGAG |
Internal bar code: | TGGTTTGACATTGCGAATTAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 129 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 19 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTAGCTTTTTCGCGCAGAG |
Suggested primer 2: | GTAAGTAGATGGGACCCGCA |