| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212132 |
| Chromosome: | chromosome 6 |
| Location: | 8980951 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311650 | IRK1 | (1 of 1) PTHR11767//PTHR11767:SF58 - INWARD RECTIFIER POTASSIUM CHANNEL // FI16807P1; Inwardly-rectifying potassium channel | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCAGAGCACACGGATCCACCACATACA |
| Internal bar code: | TTAAAGTCGCAGCGGCCCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 99.7674 |
| LEAP-Seq n confirming: | 22738 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATGAGTCTCTGACGGGT |
| Suggested primer 2: | AACACGGGAAGAAACAGGTG |