Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.212133 |
Chromosome: | chromosome 7 |
Location: | 2203850 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACGCATCAATCGGTTGATACTGCCCTG |
Internal bar code: | TTCGTCCGTGGGTCTGGACGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 818 |
LEAP-Seq percent confirming: | 93.0394 |
LEAP-Seq n confirming: | 4705 |
LEAP-Seq n nonconfirming: | 352 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAACACGGGCAATTAAACC |
Suggested primer 2: | GGCTATAGGGCTGGAGAAGG |