| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212133 |
| Chromosome: | chromosome 7 |
| Location: | 2203854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327150 | (1 of 2) IPR001810//IPR003347 - F-box domain // JmjC domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCACCGACGGGGACGTATACACACGGC |
| Internal bar code: | ACTCGATCGCGCATCCAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.6374 |
| LEAP-Seq n confirming: | 3023 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACGGGCAATTAAACC |
| Suggested primer 2: | GGCTATAGGGCTGGAGAAGG |