| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212141 |
| Chromosome: | chromosome 4 |
| Location: | 2518655 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222050 | (1 of 3) K16489 - protein SFI1 (SFI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCAAAAGCGCGCAAAGCCTCTAGTCGA |
| Internal bar code: | TAAGTGGCCTATACCGTCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 85.128 |
| LEAP-Seq n confirming: | 1929 |
| LEAP-Seq n nonconfirming: | 337 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTGTAGAATGCCCCTGC |
| Suggested primer 2: | AAACGTCGTCAACGCTCTCT |