Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.212152 |
Chromosome: | chromosome_9 |
Location: | 4152255 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393954 | FAP106 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACAAAACAAGGTTCAACCGCACGCGACCA |
Internal bar code: | TCGGAAGGTTTCGGGAAAGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 903 |
LEAP-Seq percent confirming: | 84.6866 |
LEAP-Seq n confirming: | 3661 |
LEAP-Seq n nonconfirming: | 662 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTAAAGCAGCCTTGCAACC |
Suggested primer 2: | CATTGGCACTTTCCCGTAGT |