Insertion junction: LMJ.RY0402.212152_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.212152
Chromosome:chromosome 9
Location:4152255
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393954 FAP106 Flagellar Associated Protein 106; (1 of 1) PTHR21490:SF0 - ENKURIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAAACAAGGTTCAACCGCACGCGACCA
Internal bar code:TCGGAAGGTTTCGGGAAAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:903
LEAP-Seq percent confirming:84.6866
LEAP-Seq n confirming:3661
LEAP-Seq n nonconfirming:662
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CTTAAAGCAGCCTTGCAACC
Suggested primer 2:CATTGGCACTTTCCCGTAGT