| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212152 |
| Chromosome: | chromosome_9 |
| Location: | 4152330 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g393954 | FAP106 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGTCTTGCCTCCAAACTACGCGCACGAA |
| Internal bar code: | TCTCAAGTCATGTAGCTATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.1159 |
| LEAP-Seq n confirming: | 8296 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAAAGCAGCCTTGCAACC |
| Suggested primer 2: | CAAGGCCTTAGTTCTGCCTG |