| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212174 |
| Chromosome: | chromosome 16 |
| Location: | 221926 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694703 | CGL57 | (1 of 1) PTHR12383//PTHR12383:SF5 - PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED // PEPTIDASE-S24/S26 DOMAIN-CONTAINING PROTEIN; Serine protease of S26A family | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGACACCGGACACGCCCCAGTGCCCC |
| Internal bar code: | GGCGCTGGAGTTCTTCTGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 99.8818 |
| LEAP-Seq n confirming: | 845 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCGTCATGCTTTC |
| Suggested primer 2: | TGACCAGCCCAAACTAAACC |