| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212204 |
| Chromosome: | chromosome 16 |
| Location: | 254308 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694400 | TGD2,CGL57 | Trigalactosyldiacylglycerol 2; (1 of 2) PF02470 - MlaD protein (MlaD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGTAGACGTGAAGTGTGGGGATTGTT |
| Internal bar code: | GAGTTATTCGATTTCCTAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 94.5299 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGAGCAGTGCAGGGATA |
| Suggested primer 2: | GCAGTGGTGGTAGTGTGGTG |