Insertion junction: LMJ.RY0402.212206_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.212206
Chromosome:chromosome_3
Location:2412058
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g159016 antisense CDS|outside_mRNA

Insertion site details

Flanking sequence (orientation from cassette outwards):CCAGCAGCGGTGCTTCTACGACCACCTGAG
Internal bar code:GAGTCATCGAGCCATAATTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:747
LEAP-Seq percent confirming:99.6628
LEAP-Seq n confirming:3842
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGCTCTCATAACGATGC
Suggested primer 2:GTCAAGTCCGATTGCTGGAT