Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.212206 |
Chromosome: | chromosome_3 |
Location: | 2412058 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g159016 | antisense | CDS|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGCAGCGGTGCTTCTACGACCACCTGAG |
Internal bar code: | GAGTCATCGAGCCATAATTCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 747 |
LEAP-Seq percent confirming: | 99.6628 |
LEAP-Seq n confirming: | 3842 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCGCTCTCATAACGATGC |
Suggested primer 2: | GTCAAGTCCGATTGCTGGAT |