| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212207 |
| Chromosome: | chromosome 7 |
| Location: | 1535207 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324150 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGCCTTCTGCCGGCATGGACGGAGCA |
| Internal bar code: | AACGACTAGCGTTTTTCGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.7365 |
| LEAP-Seq n confirming: | 10599 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCCACCTCCAAGTCAT |
| Suggested primer 2: | CCTTACCACAACACGCAATG |