| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212246 |
| Chromosome: | chromosome 6 |
| Location: | 2351504 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267500 | (1 of 1) K02735 - 20S proteasome subunit beta 3 (PSMB3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCTTGTTCCACGGCATTCGCGAGCGTG |
| Internal bar code: | TATCTTGCCGGTGATACGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 98.7461 |
| LEAP-Seq n confirming: | 21893 |
| LEAP-Seq n nonconfirming: | 278 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTTTCCCCAACAACCA |
| Suggested primer 2: | GCATTACGCCGCTAGAAGAC |