| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212250 |
| Chromosome: | chromosome 9 |
| Location: | 5962823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402849 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGATTGACAAGCAGTCGTACGACACACG |
| Internal bar code: | GAATCAATTCGGAGGTCTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 97.7408 |
| LEAP-Seq n confirming: | 5581 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTCCATACGCCTTCAC |
| Suggested primer 2: | TCACACTTGCTCACTCGGTC |