Insertion junction: LMJ.RY0402.212268_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.212268
Chromosome:chromosome 2
Location:6846069
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g119450 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGGTTTCAGCTTACAGCTAAGCAGGGAT
Internal bar code:TGACTGCAGGTGGTTGGCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:933
LEAP-Seq percent confirming:97.6645
LEAP-Seq n confirming:8907
LEAP-Seq n nonconfirming:213
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTCATACGCCACCAGTT
Suggested primer 2:ACACCAAGCCCTACAACGAC