Insertion junction: LMJ.RY0402.212268_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.212268
Chromosome:chromosome 2
Location:6846084
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g119450 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAAAGACATGCATCCACACCACCAGCGG
Internal bar code:ACCTGGTCGCACTCGACAAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:91.7949
LEAP-Seq n confirming:3580
LEAP-Seq n nonconfirming:320
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTCATACGCCACCAGTT
Suggested primer 2:ACACCAAGCCCTACAACGAC