| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212315 |
| Chromosome: | chromosome 8 |
| Location: | 3637347 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376350 | ASF1 | Anti-silencing factor; (1 of 1) K10753 - histone chaperone ASF1 (ASF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATCGCCGTCGCTCCGCCGTCGCTTCCC |
| Internal bar code: | CACCAGGAGTGCGGGGGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.7433 |
| LEAP-Seq n confirming: | 5439 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTCTTGCTGGATGAAG |
| Suggested primer 2: | GCTCGGGTGAGTATGTGGTT |