| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212332 |
| Chromosome: | chromosome 14 |
| Location: | 984303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614050 | (1 of 1) K16732 - protein regulator of cytokinesis 1 (PRC1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTCACCAGCTGGATCCCACAGCTGGAT |
| Internal bar code: | GATGTATACTTACAAACGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.4337 |
| LEAP-Seq n confirming: | 6321 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCATCAGCGCATCAAG |
| Suggested primer 2: | GGCTGCAGACAAGAAAGGAC |