| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212339 |
| Chromosome: | chromosome_9 |
| Location: | 290875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATTTGTGCAACCCAATCGCAGGCGACGTG |
| Internal bar code: | TAATCGCGTGCGTTGAGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.0867 |
| LEAP-Seq n confirming: | 13778 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAACTTGAGTGCGTTTC |
| Suggested primer 2: | GTACGGTAATGCTGGTGGCT |