Insertion junction: LMJ.RY0402.212344_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.212344
Chromosome:chromosome 8
Location:853337
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAATTGGCACGCATTCGATCGTCGCGCCG
Internal bar code:TTGATCGTAAAATGCATTTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:94.2029
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCGTTTGCAGTCCATTC
Suggested primer 2:CCCTTTCAGCATTTTTCCAA