| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212347 |
| Chromosome: | chromosome 2 |
| Location: | 5441879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107650 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATGAATTGGTCGTATCGACTGCGACAG |
| Internal bar code: | AGCTCGTCTCTAACTGTGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1032 |
| LEAP-Seq percent confirming: | 98.6464 |
| LEAP-Seq n confirming: | 2915 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCAAGAGTCATTCAGCA |
| Suggested primer 2: | CAGTTTAAGCTTGCGAAGGG |