Insertion junction: LMJ.RY0402.212349_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.212349
Chromosome:chromosome 13
Location:3261141
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g585950 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAACCGCCAGCAGCAGCAGTGGCAGCG
Internal bar code:GTACTGTCACGAAGCATAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1061
LEAP-Seq percent confirming:87.1045
LEAP-Seq n confirming:5424
LEAP-Seq n nonconfirming:803
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGCAATGGCTCTCACTC
Suggested primer 2:CGTCAGGCGAAACTCAAGAT