| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212354 |
| Chromosome: | chromosome 6 |
| Location: | 7004221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296750 | HYDEF,HYDEF1 | (1 of 1) PTHR11649//PTHR11649:SF59//PTHR22976//PTHR22976:SF28 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // SUBFAMILY NOT NAMED // BIOTIN SYNTHASE // SUBFAMILY NOT NAMED; Iron hydrogenase assembly protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCGCCCGGGGCCACCGCCAACCTGGAC |
| Internal bar code: | GCGGATTGCAGGGAGTGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 96.5842 |
| LEAP-Seq n confirming: | 3280 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCTGAAAGAGTTGGTC |
| Suggested primer 2: | CCACACCTCCTTCTGGTTGT |