| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212355 |
| Chromosome: | chromosome 3 |
| Location: | 8886875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207713 | ISA3 | Isoamylase, starch debranching enzyme 3; (1 of 2) 3.2.1.68 - Isoamylase / Debranching enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAAGGGGGCGGTGTTAGTGTAGGGGC |
| Internal bar code: | ACGCTAATCTCGTGGGTCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.7857 |
| LEAP-Seq n confirming: | 6052 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACATGATCGACCCCAGC |
| Suggested primer 2: | CGCACGGGATTGACTAATCT |