Insertion junction: LMJ.RY0402.212372_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.212372
Chromosome:chromosome 12
Location:697093
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g494800 DII1,IDA-IC28,IDA4,p28IDA4 (1 of 1) K10410 - dynein light intermediate chain, axonemal (DNALI); Flagellar inner arm dynein light chain p28 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTTATCCAAACGCACATAAGACGTTGTA
Internal bar code:CTGGGGCTGTTAGTAAATCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1142
LEAP-Seq percent confirming:99.2453
LEAP-Seq n confirming:526
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGATAACATCCAGGCG
Suggested primer 2:AAGGTCGACGTGAGTGTTCC