| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212388 |
| Chromosome: | chromosome 13 |
| Location: | 1177567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569700 | OPR56,MBC1 | psbC mRNA stabilization factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCCACTTGGCCCCTGCCTGCACGCGC |
| Internal bar code: | TGTCATGCGCCCGCCCTGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 98.8895 |
| LEAP-Seq n confirming: | 9528 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTTCATGGTCGCTGTC |
| Suggested primer 2: | GACCCTGCTCTGAGATCGAC |