| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212393 |
| Chromosome: | chromosome_14 |
| Location: | 920433 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613700 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTAAGACGAACCATGGGTGCATGGTGTGGG |
| Internal bar code: | GTCCCGGTATCGTCAACAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 93.4218 |
| LEAP-Seq n confirming: | 21544 |
| LEAP-Seq n nonconfirming: | 1517 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TGCTCTAGCCTGTGATGGTG |