| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212407 |
| Chromosome: | chromosome 7 |
| Location: | 2107903 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACCGGTTGCACGGGCTTTCGAGTCTT |
| Internal bar code: | GATTGGTCATAGATGTGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 96.4881 |
| LEAP-Seq n confirming: | 1099 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTTTTGTGACTGGAACT |
| Suggested primer 2: | CCACAAGTGCCACATACAGG |