Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.212423 |
Chromosome: | chromosome_13 |
Location: | 235999 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g563700 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTCCGGGCCGCCCCACGTCGTCGTTGTG |
Internal bar code: | TGAGATTAGAAGCTGGGTGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 647 |
LEAP-Seq percent confirming: | 87.1762 |
LEAP-Seq n confirming: | 673 |
LEAP-Seq n nonconfirming: | 99 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCATCTGACTCTCACGCA |
Suggested primer 2: | CACATACGCACCTGGTTGTC |