| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212436 |
| Chromosome: | chromosome 8 |
| Location: | 2026068 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368300 | HEL43 | Putative RNA helicase; (1 of 4) IPR001650//IPR006935//IPR014001//IPR027417 - Helicase, C-terminal // Helicase/UvrB, N-terminal // Helicase superfamily 1/2, ATP-binding domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGTGTTGCAGGCCCCGTGTGATGAGC |
| Internal bar code: | TTGGTTTGACTATTAAGTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 51.8328 |
| LEAP-Seq n confirming: | 8682 |
| LEAP-Seq n nonconfirming: | 8068 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGAAATGTGAATGGCG |
| Suggested primer 2: | GAGTGACGCCTTGCACTACA |