| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212437 |
| Chromosome: | chromosome 9 |
| Location: | 751479 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402450 | (1 of 1) 3.6.4.8 - Proteasome ATPase / RP triple-A protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAAAAGACAGGGCCCAGACCTGGCGCT |
| Internal bar code: | TTGCTTTTCTCCTGCAAACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 97.682 |
| LEAP-Seq n confirming: | 4509 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGAACGCTAGTTCGCC |
| Suggested primer 2: | GAAGCGACTCATCTTCCAGG |